The film featured substantially revised designs for many of the TV show's characters, with a much more angular look. Many of these changes were incorporated in the final seasons of the show, such as the Professor's new eyes and Ace's sharper teeth. The film was released in America on DVD and VHS. The DVD included extras such as deleted scenes, behind-the-scenes footage, commentaries and more. Despite being filmed in 1.85:1 aspect ratio, the DVD and VHS are in fullscreen only.
Professor Utonium hopes to create the perfect little girl using a mixture of sugar, spice, and everything nice to improve Townsville, a city plagued by villains. He is shoved by his laboratory assistant, a destructive chimpanzee named Jojo, causing him to accidentally break a container of a mysterious substance called Chemical X that spills into the mixture and explodes in Jojo's face. The Professor finds that the experiment was a success, having produced three little girls whom he names Blossom (the smart and mature one), Bubbles (the cute and bubbly one), and Buttercup (the rough and tough one). These girls also have superpowers as a result of the additional Chemical X. They all immediately grow to love each other as a family.
During their first day of school, the girls learn about the game tag and begin to play amongst themselves, which quickly grows destructive when they begin using their powers. They take their game downtown, accidentally causing massive damage to the city until the Professor calms them down and cautions them against using their powers outside. As a result of the destruction, the citizens of Townsville treat the girls as outcasts while the Professor is arrested for creating the girls. The despondent girls try to make their way home on foot, but become lost in an alleyway and are attacked by the Gangreen Gang. They are rescued by Jojo, whose brain has mutated and given him enhanced intelligence as a result of the Chemical X explosion.
Planning control of the city, Jojo gains the girls' empathy by saying he is also hated for his powers, and manipulates them into helping him build a laboratory and machine over a volcano in the middle of town that he claims will gain them the affections of the city. He also has them steal a batch of Chemical X from the Professor's lab. As a reward, Jojo takes the girls to the local zoo and secretly implants small transportation devices on all the primates there. That night, Jojo transports all the primates from the zoo into his volcano lair and uses his new machine to inject them with Chemical X, turning them into evil mutant primates like himself. The next morning, after the Professor is released from prison, the girls show him all the "good" they have done, only to discover the city being attacked by the monkeys. Jojo, renaming himself Mojo Jojo, publicly denounces the girls as his assistants, turning everyone, including the distraught Professor, against them. The girls blast off into space, dejected.
Mojo Jojo announces his intentions to rule the planet, but becomes frustrated when his minions, now as intelligent and evil as he is, begin concocting their own plans to terrorize the people of Townsville. Overhearing this turmoil from space, the girls return to Earth and use their powers to defeat the primates and rescue the citizens. In response, Mojo injects himself with Chemical X and grows into a giant monster, but the girls defeat him after an intense battle by pushing him off a skyscraper. Hoping to help the girls, the Professor develops an antidote for Chemical X which Mojo Jojo lands on, shrinking him down to his original size. The girls consider using the Antidote X to erase their powers, thinking they would be accepted as normal little girls, but the citizens of Townsville protest, apologizing for misjudging the girls and thanking them for their heroic deeds. At the insistence of the Mayor, the girls agree to use their powers to defend Townsville and become the city's beloved crime-fighting team of superheroes: the Powerpuff Girls.
|Mojo Jojo||Roger L. Jackson|
|Professor Utonium||Tom Kane|
|Mayor, Narrator, Li'l Arturo, Snake, Cha-Ching Cha-Ching, Mitch Mitchelson||Tom Kenny|
|Ms. Keane||Jennifer Hale|
|Sarah Bellum||Jennifer Martin|
|Ace, Big Billy, Grubber, Baboon Kaboom, Go-Go Patrol, Hacha Chacha||Jeff Bennett|
|Linda, Woman at Zoo||Grey DeLisle|
|I.P. Host, Local Anchor||Phil LaMarr|
|Hota Wata, Killa Drilla, Blah-Blah Blah-Blah, The Doot Da Doot Da Doo Doos||Rob Paulsen|
|Rocko Socko, Ojo Tango||Kevin Michael Richardson|
| Pappy Wappy, Tonsa Muncha, Rolla Ova, Bongo Bango, Whacko Smacko,|
Unnamed Newscaster, Screaming Telephone Man
|Dee Bradley Baker|
|Various Monkeys||Frank Welker|
- ↑ 1.0 1.1 "The Powerpuff Girls Movie TALKBACK ". toonzone.net. March 5, 2004. http://www.toonzone.net/forums/threads/the-powerpuff-girls-movie-talkback-spoilers.3573641/. Retrieved on November 14, 2017.